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Ryuzo Yanagimachi has made seminal contributions toward understanding the process and mechanism of mammalian fertilization. He is a pioneer of assisted fertilization technologies such as in vitro fertilization and direct sperm injection into egg which are widely used today in human infertility clinics throughout the world. He was also a pioneer in the cloning field. In 1997 his laboratory at the University of HawaiÊ»i at MÄnoa successfully cloned mice using the Honolulu technique. The first one was a female named Cumulina from the cells that surround the developing ovarian follicle in mice.
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